中文名 | H33258荧光染料 |
英文名 | BBIH |
别名 | 双苯咪唑 甲嗪双苯咪酚 33258荧光染料 H33258荧光染料 HOECHST 33258 荧光染料 HOECHST 33258细胞核染料 HOECHST 33258细胞蓝色荧光染料 HOECHST 33258细胞核染料*20MM水溶液* |
英文别名 | BBIH Pibenzimol HCl Bis-BenzimideFluorochrome Phenol, p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2-benzimidazolyl)-, trihydrochloride p-(5-(5-(4-Methyl-1-piperazinyl)-1H-2-benzimidazolyl)-1H-2-benzimidazolyl)phenol trihydrochloride bisBenzimide, 2μ-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi-1H-benzimidazole hydrate trihydrochloride 4-[5-[6-(4-Methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride HOE 33258, Hoechst 33258, 2μ-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride |
CAS | 23491-45-4 |
EINECS | 245-690-6 |
化学式 | C25H25ClN6O |
分子量 | 460.97 |
密度 | 1.5099 (rough estimate) |
熔点 | 314 °C |
水溶性 | SOLUBLE |
溶解度 | H2O: 10 mg/mL |
折射率 | 1.6300 (estimate) |
存储条件 | -20°C |
外观 | 粉末 |
颜色 | Yellow to Very Dark Yellow |
BRN | 4088183 |
MDL号 | MFCD00012679 |
体外研究 | Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC 50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI 50 from 84×10 -6 to 191.5×10 -6 mol/dm 3 . Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1. |
危险品标志 | Xn - 有害物品 |
风险术语 | R22 - 吞食有害。 R36/38 - 刺激眼睛和皮肤。 R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 R20/21/22 - 吸入、皮肤接触及吞食有害。 |
安全术语 | S26 - 不慎与眼睛接触后,请立即用大量清水冲洗并征求医生意见。 S36 - 穿戴适当的防护服。 S24/25 - 避免与皮肤和眼睛接触。 S23 - 切勿吸入蒸汽。 |
WGK Germany | 3 |
RTECS | SM1140500 |
FLUKA BRAND F CODES | 3-8-10 |
海关编号 | 32129000 |
参考资料 展开查看 | 1. 郭碧汝,佟瑶,董岩,袁橙,钱佳怡,韩翠艳.红细胞膜包覆葫芦素B纳米结构脂质载体的制备及体外抗乳腺癌作用评价[J].国际药学研究杂志,2020,47(11):989-994+1000. 2. [IF=6.986] Xu Jie et al."Red–Near-Infrared Fluorescent Probe for Time-Resolved in Vivo Alkaline Phosphatase Detection with the Assistance of a Photoresponsive Nanocontainer."Anal Chem. 2019;91(20):13174–13182 |
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